Dynamic Regulation of Biofilm Formation and Metabolic Processes in Bacillus cereus Under Different Culture Conditions

Bacillus cereus is a significant pathogen responsible for toxin-induced foodborne diseases, and is widely distributed in the natural environment. B. cereus biofilm formation is regulated by multifactorial interactions, demonstrating dynamic adaptability to environmental cues. In this study, crystal violet staining and MTT staining methods were employed to conduct an in-depth investigation into the biofilm formation and metabolic activity changes of five strains of B. cereus in different culture media and with various culture conditions. Experimental data indicated that the composition of the culture medium, the length of incubation time, and the fluctuation of temperature all had a significant impact on the biofilm formation of B. cereus, and this impact exhibited obvious differences among different strains. This result provides a solid experimental basis for deeply elucidating the internal regulatory mechanism of B. cereus biofilm formation and the change principles of metabolic activity.